Looping and interaction between hypersensitive sites in the active beta-globin locus. Tolhuis, B., Palstra, R.J., Splinter, E., Grosveld, F. 3C technology: analyzing the spatial organization of genomic loci in vivo. Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. Mapping chromatin interactions by Chromosome Conformation Capture (3C). The three C's of chromosome conformation capture: controls, controls, controls. Does looping and clustering in the nucleus regulate gene expression? Curr. Spatial organization of gene expression: the active chromatin hub. A closer look at long-range chromosomal interactions. Interchromosomal interactions and olfactory receptor choice. Long-range control of gene expression: emerging mechanisms and disruption in disease. The 5C protocol described here can be completed in 13 d.ĮNCODE-consortium The ENCODE (ENCyclopedia Of DNA Elements) project. The resulting 5C library of ligated primers is analyzed using either microarray detection or ultra-high-throughput DNA sequencing. The 5C technology then employs highly multiplexed ligation-mediated amplification (LMA) to detect and amplify 3C ligation junctions. Ligation products are subsequently purified to generate a 3C library. The chromatin is solubilized, digested with a restriction enzyme and ligated at low DNA concentration to promote intra-molecular ligation of cross-linked DNA fragments. As in 3C, cells are treated with formaldehyde to cross-link chromatin interactions. We recently developed a high-throughput adaptation of 3C, 3C-carbon copy (5C), that is used to measure networks of millions of chromatin interactions in parallel.
Interactions between genomic elements can be detected using the chromosome conformation capture (3C) technology. Genomic elements separated by large genomic distances can physically interact to mediate long-range gene regulation and other chromosomal processes.
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